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Evaluation of column flotation in the downstream processing of fermentation products: recovery of a genetically engineered α-amylase
Michigan State University.
1993 (English)In: Biotechnology progress (Print), ISSN 8756-7938, E-ISSN 1520-6033, Vol. 9, no 4, p. 411-420Article in journal (Refereed) Published
Abstract [en]

Flotation is a simple, inexpensive, and versatile unit operation with a largely unexplored potential in biotechnology. There is a general lack of research concerning biotechnological applications in this area, especially in the recovery of fermentation products. Moreover, the few reports in the literature do not consider the modern concept of column flotation as practiced in the mineral industry. We report herein the application of column flotation for the recovery of a Bacillus stearothermophilus α-amylase expressed in Escherichia coli by the use of a food-grade polymer, (hydroxypropyl)methylcellulose (HPMC), and ammonium sulfate. First, the enzyme was removed from the liquid phase by partition to a salted-out HPMC phase. The enzyme-containing polymer flocs were then floated from the liquid. Recovery of active enzyme was as high as 90%, with throughput as high as 94 m3/(h·m2). The floatability of the enzyme from a periplasmic extract was higher than extracellular enzyme in the broth due to the presence of depressors of molecular weight lower than 10 000 in the broth.

Place, publisher, year, edition, pages
1993. Vol. 9, no 4, p. 411-420
National Category
Bioprocess Technology
Research subject
Biochemical Process Engineering
Identifiers
URN: urn:nbn:se:ltu:diva-6703DOI: 10.1021/bp00022a008Local ID: 4f9dfeb0-3bd9-11de-a8ec-000ea68e967bOAI: oai:DiVA.org:ltu-6703DiVA, id: diva2:979589
Note
Upprättat; 1993; 20090508 (ysko)Available from: 2016-09-29 Created: 2016-09-29 Last updated: 2017-11-24Bibliographically approved

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Berglund, Kris

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