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Identification of features associated with plant cell wall recalcitrance to pretreatment by alkaline hydrogen peroxide in diverse bioenergy feedstocks using glycome profiling
Michigan State University.
University of Georgia.
University of Georgia.
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Sustainable Process Engineering.ORCID iD: 0000-0002-9313-941X
2014 (English)In: RSC Advances, ISSN 2046-2069, E-ISSN 2046-2069, Vol. 4, no 33, p. 17282-17292Article in journal (Refereed) Published
Abstract [en]

A woody dicot (hybrid poplar), an herbaceous dicot (goldenrod), and a graminaceous monocot (corn stover) were subjected to alkaline hydrogen peroxide (AHP) pretreatment and subsequent enzymatic hydrolysis in order to assess how taxonomically and structurally diverse biomass feedstocks respond to a mild alkaline oxidative pretreatment and how differing features of the cell wall matrix contribute to its recalcitrance. Using glycome profiling, we determined changes in the extractability of non-cellulosic glucans following pretreatment by screening extracts of the pretreated walls with a panel of 155 cell wall glycan-directed monoclonal antibodies to determine differences in the abundance and distribution of non-cellulosic glycan epitopes in these extracts and assess pretreatment-induced changes in the structural integrity of the cell wall. Two taxonomically-dependent outcomes of pretreatment were identified that both improved the subsequent enzymatic hydrolysis yields but differed in their impacts on cell wall structural integrity. Specifically, it was revealed that goldenrod walls exhibited decreases in all classes of alkali-extractable glycans indicating their solubilization during pretreatment, which was accompanied by an improvement in the subsequent extractability of the remaining cell wall glycans. The corn stover walls did not show the same decreases in glycan abundance in extracts following pretreatment, but rather mild increases in all classes of cell wall glycans, indicating overall weaker associations between cell wall polymers and improved extractability. The hybrid poplar walls were relatively unaffected by pretreatment in terms of composition, enzymatic hydrolysis, and the extractability of cell wall glycans due presumably to their higher lignin content and denser vascular structure.

Place, publisher, year, edition, pages
2014. Vol. 4, no 33, p. 17282-17292
National Category
Bioprocess Technology
Research subject
Biochemical Process Engineering
Identifiers
URN: urn:nbn:se:ltu:diva-7382DOI: 10.1039/c4ra00824cISI: 000335226700057Scopus ID: 2-s2.0-84938751692Local ID: 5c15fed6-1c38-408a-a6da-de8770f90b7bOAI: oai:DiVA.org:ltu-7382DiVA, id: diva2:980271
Note
Validerad; 2014; 20140602 (johsod)Available from: 2016-09-29 Created: 2016-09-29 Last updated: 2018-07-10Bibliographically approved

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