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Near field optical microscopy in aqueous solution: implementation and characterization of a vibrating probe
Luleå tekniska universitet.
University of Virginia School of Medicine.
University of Virginia School of Medicine.
University of Virginia School of Medicine.
2002 (English)In: Journal of Microscopy, ISSN 0022-2720, E-ISSN 1365-2818, Vol. 205, no 1, p. 53-60Article in journal (Refereed) Published
Abstract [en]

Near field optical microscopy (NSOM) is one of the possible solutions to circumvent the diffraction limit, but the control of the optical probe in solution has been a technical challenge for practical applications. Most recently, it has been shown that the pipette used in the scanning ion conductance microscope can be modified to form a high resolution near field optical probe. When combined with a novel distance modulation mechanism, a robust near field microscope can be constructed for operation in aqueous solution. In this paper, we present technical details of this design and a further characterization of the NSOM system for imaging in solution. Fundamental limitations of this approach in comparison to other systems are also discussed. Based on the current technology, it is concluded that better than 50 nm resolution should be achievable with this technique for fluorescence, as well as fluorescence resonance energy transfer, imaging of biological specimens.

Place, publisher, year, edition, pages
2002. Vol. 205, no 1, p. 53-60
National Category
Chemical Process Engineering
Research subject
Chemical Technology
Identifiers
URN: urn:nbn:se:ltu:diva-7544DOI: 10.1046/j.0022-2720.2001.00965.xISI: 000173685600007Scopus ID: 2-s2.0-0036165177Local ID: 5ef52320-e75d-11db-8a98-000ea68e967bOAI: oai:DiVA.org:ltu-7544DiVA, id: diva2:980434
Note

Validerad; 2002; 20061106 (ysko)

Available from: 2016-09-29 Created: 2016-09-29 Last updated: 2018-07-10Bibliographically approved

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