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Immobilization of horseradish peroxidase on β-cyclodextrin-capped silver nanoparticles: Its future aspects in biosensor application
Luleå University of Technology, Department of Engineering Sciences and Mathematics, Material Science.
School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi.
School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi.
School of Chemical Sciences, Universiti Sains Malaysia, Minden Penang.
2016 (English)In: Preparative Biochemistry & Biotechnology, ISSN 1082-6068, E-ISSN 1532-2297, Vol. 46, no 4, p. 321-327Article in journal (Refereed) Published
Abstract [en]

This study aimed to work out a simple and high-yield procedure for the immobilization of horseradish peroxidase on silver nanoparticle. Ultraviolet–visible (UV-vis) and Fourier-transform infrared spectroscopy and transmission electron microscopy were used to characterize silver nanoparticles. Horseradish peroxidase was immobilized on β-cyclodextrin-capped silver nanoparticles via glutaraldehyde cross-linking. Single-cell gel electrophoresis (Comet assay) was also performed to confirm the genotoxicity of silver nanoparticles. To decrease toxicity, silver nanoparticles were capped with β-cyclodextrin. A comparative stability study of soluble and immobilized enzyme preparations was investigated against pH, temperature, and chaotropic agent, urea. The results showed that the cross-linked peroxidase was significantly more stable as compared to the soluble counterpart. The immobilized enzyme exhibited stable enzyme activities after repeated uses.

Place, publisher, year, edition, pages
2016. Vol. 46, no 4, p. 321-327
National Category
Bio Materials
Research subject
Wood and Bionanocomposites
Identifiers
URN: urn:nbn:se:ltu:diva-7633DOI: 10.1080/10826068.2015.1031389ISI: 000377930000001PubMedID: 25830286Scopus ID: 2-s2.0-84975842559Local ID: 60853416-75c7-4478-9d51-56e0f91d9b82OAI: oai:DiVA.org:ltu-7633DiVA, id: diva2:980523
Note

Validerad; 2016; Nivå 2; 20160613 (andbra)

Available from: 2016-09-29 Created: 2016-09-29 Last updated: 2018-07-10Bibliographically approved

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Karim, Zoheb

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