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Raman spectroscopy as a tool for detecting mitochondrial fitness
CBZI, University of Nova Gorica, Vipava.
CBZI, University of Nova Gorica, Vipava.
Luleå University of Technology, Department of Engineering Sciences and Mathematics, Fluid and Experimental Mechanics.
Number of Authors: 3
2016 (English)In: Journal of Raman Spectroscopy, ISSN 0377-0486, E-ISSN 1097-4555, Vol. 47, no 8, p. 933-939Article in journal (Refereed) Published
Abstract [en]

Raman spectroscopy allows the molecular chemical analysis of whole living cells by comparing them to known Raman signatures of specific vibrational bonds. In this work we used Raman spectroscopy to differentiate between wild type yeast cells and mutants characterized by increased or reduced mitochondrial fragmentation. To associate mitochondrial fragmentation with biochemical markers, we performed Linear Discriminant Analysis (LDA) of whole cell Raman spectra (~50–100 cells/spectrum). We show that the long-lived, less fragmented mutants fall into a significantly distant cluster from the wild type and short-lived, more fragmented mutants. Clustering depends on respiratory growth and coincides with that of membrane phospholipids and some respiratory chain components. Spectral clustering is supported by enzymatic activity measurements of OXPHOS Complexes. In addition, we find that NAD(P)H autofluorescence also correlates with mitochondrial fragmentation, representing another likely aging biomarker, besides phospholipids and OXPHOS components. In summary, we demonstrate that Raman spectroscopy has the potential to become a powerful tool for differentiating healthy from unhealthy aged tissues, as well as for the prognostic evaluation of mitochondrial function and fitness.

Place, publisher, year, edition, pages
2016. Vol. 47, no 8, p. 933-939
National Category
Applied Mechanics
Research subject
Experimental Mechanics
Identifiers
URN: urn:nbn:se:ltu:diva-7725DOI: 10.1002/jrs.4930ISI: 000380949500009Local ID: 624a7da3-d98b-4a00-a755-02ee20e082cfOAI: oai:DiVA.org:ltu-7725DiVA: diva2:980615
Note

Validerad; 2016; Nivå 2; 20160419 (andbra)

Available from: 2016-09-29 Created: 2016-09-29 Last updated: 2017-11-24Bibliographically approved

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