Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Lignin boosts the cellulase performance of a GH-61 enzyme from Sporotrichum thermophile
Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens.
Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens.
Chalmers University of Technology.
2012 (English)In: Bioresource Technology, ISSN 0960-8524, E-ISSN 1873-2976, Vol. 110, p. 480-487Article in journal (Refereed) Published
Abstract [en]

An enzyme belonging to the glycoside hydrolase family 61 from the thermophilic fungus Sporotrichum thermophile, was functionally expressed in the methylotrophic yeast Pichia pastoris under the transcriptional control of the alcohol oxidase (AOX1) promoter. The enzyme hydrolyzed barley β-glucan, carboxymethyl cellulose, lichenan, wheat arabinoxylan and birchwood xylan showing optimal activity at pH 8 and 65 °C. A 2:1 mixture of Celluclast 1.5 L and StCel61a was capable of increasing the degree of spruce conversion by 42%. The use of substrates with varying lignin content permitted the detection of a dependence of the enhancing capacity of StCel61a on the radical scavenging capacity of the different lignocellulosics. In the presence of a reductant, StCel61a boosted the efficiency of a mixture of purified cellulases (EGII, CBHI, β-GLUC) by 20%. The synergistic activity exhibited by StCel61a and its dependence on reducing substances provide guidelines for process design towards the production of economically viable bioethanol.

Place, publisher, year, edition, pages
2012. Vol. 110, p. 480-487
National Category
Bioprocess Technology
Identifiers
URN: urn:nbn:se:ltu:diva-7847DOI: 10.1016/j.biortech.2012.01.116Local ID: 64451a2f-8278-4739-bd82-fe0d14de1d31OAI: oai:DiVA.org:ltu-7847DiVA, id: diva2:980737
Note
Upprättat; 2012; 20130212 (ysko)Available from: 2016-09-29 Created: 2016-09-29 Last updated: 2017-11-24Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full text

Authority records BETA

Christakopoulos, Paul

Search in DiVA

By author/editor
Christakopoulos, Paul
In the same journal
Bioresource Technology
Bioprocess Technology

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 45 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf