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Soluble and insoluble solids contributions to high-solids enzymatic hydrolysis of lignocellulose
Texas Tech University, Lubbock.
National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO.
National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO.
2008 (English)In: Bioresource Technology, ISSN 0960-8524, E-ISSN 1873-2976, Vol. 99, no 18, p. 8940-8948Article in journal (Refereed) Published
Abstract [en]

The rates and extents of enzymatic cellulose hydrolysis of dilute acid pretreated corn stover (PCS) decline with increasing slurry concentration. However, mass transfer limitations are not apparent until insoluble solids concentrations approach 20% w/w, indicating that inhibition of enzyme hydrolysis at lower solids concentrations is primarily due to soluble components. Consequently, the inhibitory effects of pH-adjusted pretreatment liquor on the enzymatic hydrolysis of PCS were investigated. A response surface methodology (RSM) was applied to empirically model how hydrolysis performance varied as a function of enzyme loading (12-40 mg protein/g cellulose) and insoluble solids concentration (5-13%) in full-slurry hydrolyzates. Factorial design and analysis of variance (ANOVA) were also used to assess the contribution of the major classes of soluble components (acetic acid, phenolics, furans, sugars) to total inhibition. High sugar concentrations (130 g/L total initial background sugars) were shown to be the primary cause of performance inhibition, with acetic acid (15 g/L) only slightly inhibiting enzymatic hydrolysis and phenolic compounds (9 g/L total including vanillin, syringaldehyde, and 4-hydroxycinnamic acid) and furans (8 g/L total of furfural and hydroxymethylfurfural, HMF) with only a minor effect on reaction kinetics. It was also demonstrated that this enzyme inhibition in high-solids PCS slurries can be approximated using a synthetic hydrolyzate composed of pure sugars supplemented with a mixture of acetic acid, furans, and phenolic compounds, which indicates that generally all of the reaction rate-determining soluble compounds for this system can be approximated synthetically.

Place, publisher, year, edition, pages
2008. Vol. 99, no 18, p. 8940-8948
National Category
Bioprocess Technology
Research subject
Biochemical Process Engineering
Identifiers
URN: urn:nbn:se:ltu:diva-8985DOI: 10.1016/j.biortech.2008.05.015ISI: 000259888700063PubMedID: 18585030Scopus ID: 2-s2.0-50349090462Local ID: 78a12cb0-7366-11dd-a60f-000ea68e967bOAI: oai:DiVA.org:ltu-8985DiVA, id: diva2:981923
Note
Validerad; 2008; 20080826 (ysko)Available from: 2016-09-29 Created: 2016-09-29 Last updated: 2018-07-10Bibliographically approved

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