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A novel lipolytic activity of Rhodotorula glutinis cells: Production, partial characterization and application in the synthesis of esters
National Technical University of Athens.
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1999 (English)In: Journal of Bioscience and Bioengineering, ISSN 1389-1723, E-ISSN 1347-4421, Vol. 88, no 1, p. 53-56Article in journal (Refereed) Published
Abstract [en]

Cell-bound lipase activity (10 pNPL units/g dry cell weight) was released when the yeast Rhodotorula glutinis was cultured in a 7-l stirred tank fermentor using palm-oil as the sole carbon source. The enzyme showed relative specificity towards medium chain organic acids since the apparent Km values for pNPB (p-NitroPhenyl-Butyrate) and pNPL (p-NitroPhenyl-Laurate) were equal to 2.7 and 0.7 mM, respectively. In addition, 80% of this activity could be detected on the surface of the cells. The cell-bound nature of the enzyme increased its thermal stability showing half-life times of 200 and 60 min at 50 and 60°C, respectively, as well as good stability in organic solvents. Freeze-dried cell preparations were successfully used to catalyze the synthesis of fatty acid esters of butanol and heptanol in nearly anhydrous organic solvents. A conversion of 60–62% was obtained upon esterification of palmitic or oleic acid with butanol, within 96 h. The enzyme preparation was used in four consecutive batch reactions with only 10% loss of activity.

Place, publisher, year, edition, pages
1999. Vol. 88, no 1, p. 53-56
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URN: urn:nbn:se:ltu:diva-9480DOI: 10.1016/S1389-1723(99)80175-3Local ID: 81fa699a-bf6c-4992-a89e-9885ca6480ebOAI: oai:DiVA.org:ltu-9480DiVA, id: diva2:982418
Note
Upprättat; 1999; 20130218 (ysko)Available from: 2016-09-29 Created: 2016-09-29 Last updated: 2017-11-24Bibliographically approved

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Christakopoulos, Paul

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