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Purification and characterisation of NAD+-dependent xylitol dehydrogenase from Fusarium oxysporum
Department of Chemical Engineering, National Technical University of Athens.
Department of Chemical Engineering, National Technical University of Athens.
Department of Chemical Engineering, National Technical University of Athens.
2002 (English)In: Biotechnology letters, ISSN 0141-5492, E-ISSN 1573-6776, Vol. 24, no 24, p. 2089-2092Article in journal (Refereed) Published
Abstract [en]

An NAD+-dependent xylitol dehydrogenase (XDH) from Fusarium oxysporum, a key enzyme in the conversion of xylose to ethanol, was purified to homogeneity and characterised. It was homodimeric with a subunit of Mr 48 000, and pI 3.6. It was optimally active at 45°C and pH 9-10. It was fully stable at pH 6-7 for 24 h and 30°C. Km values for D-xylitol and NAD+ were 94 mM and 0.14 mM, respectively. Mn2+ at 10 mM increased XDH activity 2-fold and Cu2+ at 10 mM inhibited activity completely.

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2002. Vol. 24, no 24, p. 2089-2092
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URN: urn:nbn:se:ltu:diva-10943DOI: 10.1023/A:1021317614948Local ID: 9d634284-8114-4ecd-987b-2185be6d4236OAI: oai:DiVA.org:ltu-10943DiVA, id: diva2:983891
Note
Upprättat; 2002; 20130213 (cani)Available from: 2016-09-29 Created: 2016-09-29 Last updated: 2017-11-24Bibliographically approved

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