The purpose of this study was to investigate the specific role of
leucine in the stimulation of the mammalian target of rapamycin
signalling pathway. Six male subjects performed four heavy
resistance exercise sessions, each separated by approximately one
week. Subjects were randomly supplemented with one of four
drinks: placebo (flavored water), leucine or essential amino acids
(EAA) with and without leucine. Immediately following each
exercise session, four subjects were infused with a flooding dose of
L-[2H5] phenylalanine (Inf) while two subjects served as controls
(Ctrl). Muscle biopsies were taken before and one hour after
exercise. In the Ctrl group, resistance exercise resulted in a
substantial increase (45-fold) in p70 kinase phosphorylation
when all EAA were ingested, whereas ingestion of leucine alone
had no greater effect than that of placebo. In the Inf group,
however, ingestion of leucine alone and EAA increased p70
phosphorylation to a similar extent (35-fold). The divergent
signalling response in the two groups suggests that leucine alone is
insufficient to increase p70
phosphorylation. Indeed, in the Inf
group, there was a strong correlation (r=0.91) between
p70 phosphorylation and the product of muscle leucine and
phenylalanine levels. These results suggest that the stimulatory
effect of leucine on p70 phosphorylation is dependent on
elevated muscle phenylalanine levels. Supported by the Swedish
National Centre for Research in Sports