The development of assistive systems and intelligent transport systems (ITS) plays an important role for improving the safety of powered two-wheelers (PTWs). Assistive systems for cars are well known and increasingly popular but for PTW riders the development of Advanced Rider Assistance Systems (ARAS) and On-Bike Information Systems (OBIS) has not progressed far enough yet. Estimates suggest that population-wide deployment of ARAS could reduce crashes by up to 40% (Lenné et al., 2011). Within the 2BESAFE project the factors that affect the acceptance of ARAS and OBIS of PTW riders and the obstacles that may hold PTW riders off from the use of assistive systems have been identified. A literature review, focus group interviews and an online survey have been conducted. The results show that the acceptability of systems depends on their function. The acceptability is higher for systems that were perceived to be more useful in emergencies. Survey respondents raised several concerns regarding the acceptance of assistive systems for PTWs. Respondents of the on-line survey felt that there was too much focus on assistive systems as a means of improving PTW rider safety, and less on the dangers that motorcyclists face actually from the actions of other road users.