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Circular economy approach to eggshell waste utilisation: Insoluble protein extraction and CaCO3 upcycling for carbonated hydroxyapatite (cHAP)-based fire-resistant wood
Division of Building Materials, Department of Building and Environmental Technology, Faculty of Engineering, LTH, Lund University, Lund, Sweden; Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Vilnius, Lithuania.ORCID iD: 0000-0003-3283-9313
Institute of Biological Chemistry, Biophysics and Bioengineering, School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh, United Kingdom; Department of Physics, Toronto Metropolitan University, Toronto, Canada.
Faculty of Materials Science and Technology in Trnava, Slovak University of Technology in Bratislava, Trnava, Slovakia.ORCID iD: 0000-0002-0060-5785
Faculty of Materials Science and Technology in Trnava, Slovak University of Technology in Bratislava, Trnava, Slovakia.
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2026 (English)In: PLOS ONE, E-ISSN 1932-6203, Vol. 21, no 6, article id e0351943Article in journal (Refereed) Published
Abstract [en]

Transitioning to a resource-efficient and sustainable circular economy is vital for tackling climate- and environmental-related challenges. This study demonstrates a closed-loop strategy for upcycling agricultural biowaste eggshells. Water- insoluble proteins were extracted from both shell membranes and shell fragments by boiling in water using protein denaturants. The ground eggshells also were used to prepare calcium acetate (Ca(CH3COO)2) and to treat Scots pine (Pinus sylvestris L.) sapwood. Mineralisation of the wood was achieved by performing a two-step impregnation process using aqueous solutions of ammonium dihydrogen phosphate (NH4H2PO4) and Ca(CH3COO)2 salts. Morphological studies revealed the relatively low saturation of wood matrix with mineral, with cell lumina mostly unfilled, while elemental mapping confirmed homogeneous distribution of Ca and P within the wood matrix. Powder X-ray diffraction (XRD) analysis revealed that wood treatment resulted in the in-situ co-precipitation of low-crystallinity hydroxyapatite (Ca10(PO4)6(OH)2), and spectroscopic analysis indicated carbonate substitution within the Ca10(PO4)6(OH)2 crystal lattice, suggesting the formation of carbonated hydroxyapatite (Ca10-x(PO4)6-x(CO3)x(OH)2-x-2y(CO3)y). Microscale combustion calorimeter (MCC) and cone calorimeter (CC) measurements of mineralised wood revealed a reduction in the total heat release (THR) compared with untreated wood, indicating potential for further optimisation of wood modification process. Results suggest that the proposed aqueous solution-based processing approach for converting an abundant resource, chicken eggshells, into value-added products has potential for new technology and bioeconomy development and represents a promising pathway towards improved sustainability.

Place, publisher, year, edition, pages
Public Library of Science (PLoS), 2026. Vol. 21, no 6, article id e0351943
National Category
Wood Science
Research subject
Fire Technology; Wood Science and Engineering
Identifiers
URN: urn:nbn:se:ltu:diva-118892DOI: 10.1371/journal.pone.0351943OAI: oai:DiVA.org:ltu-118892DiVA, id: diva2:2081001
Funder
Brandforsk, 321-006 (2022)
Note

Funder: Slovak Research and Development Agency (APVV-24-0143); VEGA (1/0755/25);

Full text license: CC BY

Available from: 2026-06-29 Created: 2026-06-29 Last updated: 2026-06-29Bibliographically approved

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Försth, MichaelBuck, DietrichSandberg, Dick

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3132333435363734 of 91
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