Role stress has been studied in business administration and social psychology in general, with limited attention to the entrepreneurship context. This paper defines entrepreneur role stress from a literature review and empirically examines the structure and homogeneity of a proposed construct and its properties in a survey directed to 1,500 entrepreneurs. Factor analysis reveals entrepreneur role stress as being consistent with the three facets proposed - role conflict, role ambiguity and role overload. Further, homogeneity tests indicate that the structure of role stress is not significantly different between subgroups of entrepreneurs (serial vs. novice entrepreneurs, push vs. pull entrepreneurs), implying the usability of role stress among entrepreneurs.