Force interactions between native cellulose microsphere (CM), sulfate cellulose microsphere (SCM), phosphate cellulose microsphere (PCM) and glass substrate under the effect of silver ions in the model solution were investigated using atomic force microscopy (AFM). The special interest was the influence of Ag+ ions and pH on the interaction between the probe and glass substrate. The probe was functionalized with above three types of cellulose microspheres by gluing method using contact mode with AFM. Functionalized probe and glass substrate were immersed in aqueous Ag+ solutions (50mg/L) at various pH value (4.25, 5.07, 5.62 and 6.63). Zeta potential studies showed negatively charged surfaces for CM, SCM, PCM and silica powders in model Ag+ solutions. The analysis revealed a decrease of adhesion force for CM and SCM system but an increase of adhesion force for PCM system with the increase of pH. The possible mechanism of the interaction under the effects of Ag+ ions, pH, and functional group contents as well as the shape of the cellulose microspheres was discussed. The SEM and SEM-EDS analysis confirmed the presence of silver ions on the surface of the cellulose microspheres after adsorption and force measurements.