A stiffness sensitive sensor capable of measuring the stiffness of a soft object through contact has been implemented through the resonance sensor technique. This technique is based on a piezoelectric element in a feedback circuit configuration. At contact with a soft tissue the resonance frequency changes and together with force measurement it is possible to estimate the elastic stiffness of the measured object. Earlier sensor implementations have been limited to controlled indentation setups. Recently, the hand-held resonance sensor concept was introduced and evaluated on a soft tissue phantom. The aim of this study was to investigate the concept on soft biological tissue, specifically to investigate if the measured stiffness was impression speed independent. Measurements were conducted on porcine muscle tissue and a stiffness parameter and an impression speed parameter were calculated. Correlation analysis showed weak non-significant correlation suggesting that the stiffness was independent of impression speed. This was promising for further studies with the handheld resonance sensor on soft biological tissue.